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Introducing HyParComp (8)

Unlock the power of flow cytometry with fluorescence controls that provide workflow efficiency.

Flow cytometry today requires precise controls for accurate data. SpectraComp and HyParComp are designed to support easier analysis workflows with their cell-like attributes (size, scatter, and autofluorescence).

Stay ahead of the game with cutting-edge controls designed to provide reliable results you can trust.

  • Precisely Mimic Cell Attributes
  • Unparalleled Brightness
  • Accurate Compensation and Unmixing Calculations

Spectral Unmixing

SpectraComp® are single stain controls that optimize spectral unmixing results to reduce errors associated with spectral overlap. 

  • SpectraComp® unmixing controls engineered for performance in spectral analysis and unmixing
  • SpectraComp® XT: now with 5 species capability
    (see chart below!)
Learn More

Compensation 

HyParComp™ are single stain controls that optimize compensation results to reduce errors associated with spectral overlap. 

  • HyParComp™ compensation or unmixing controls that optimize workflow efficiency 

  • HyParComp™ XT: now with 5 species capability 
    (see chart below!)
Learn More

Fluorescent Protein

Controls

SpectraComp® eGFP and mCherry are multi-level fluorescent controls for unmixing, compensation, and benchmarking.
  • State-of-the-art control designed using actual eGFP 
  • Three mean fluorescence intensity levels
    (low, medium, and high)
  • Consistent fluorescence signals for reliable reference
Learn More
eGFP histogram
HyParComp XT Rabbit
HyParCmp XT human
Autofluorescences in Spectral Analysis SpectraComp

SpectraComp® are single stain controls that optimize spectral unmixing results to reduce errors associated with spectral overlap. These controls mimic the staining performance of real cells.

eGFP positive spectral profile


SpectraComp® and HyParComp™ Comparison 

Antibody Origin SpectraComp® HyParComp™ SpectraComp® XT HyParComp™ XT SpectraComp® eGFP

Mouse

 + + + +  
Rat + + + +  
Hamster + + + +  
Rabbit     + +  
Human     + +  
eGFP Multi-Level         +

 

 

Commercial Tutorial 2023 (1)

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Frequently asked questions

What's the difference between HyParComp™ and SpectraComp®

HyParComp™ is optimized for conventional flow cytometry. HyParComp™ are single stain controls that optimize compensation results to reduce errors associated with spectral overlap. These cell mimics are intended as compensation controls to match the single-staining performance of real cells. This product has positive and negative populations separated into different bottles rather than mixed, as with SpectraComp®.

SpectraComp® are single stain controls that optimize spectral unmixing results to reduce errors associated with spectral overlap. This product is intended for spectral mixing or conventional compensation to match the single staining of real cells. Staining with SpectraComp® yields a positive and negative fluorescence peak that will aid in resolving spectral overlap during spectral unmixing or compensation. This product comes in one vial with negative and positive capture mimics combined.

How does HyParComp™ compare to other compensation beads or cells?

HyParComp™ are extremely bright mimics with a larger binding capacity than polystyrene beads. The binding capacity in HyParComp™ is generally greater than cells, so you will always have enough of the bright population(s) you will need for compensation, unlike cells whose expression of markers can be absent, varied, or unpredictable. HyParComp™also has an autofluorescence that more closely mimics that of lymphocytes/monocytes compared to polystyrene beads, which yields advantages over polystyrene beads when using spectral unmixing or spectral compensation.

What instruments can I run HyParComp™ on?

HyParComp™ can be run on most flow analyzers. HyParComp™ can be run on sorters with low PSI (less than 35PSI).

What is the XT?

XT is the naming mechanism to reflect all 5 species on either SpectraComp or HyParComp products. Think XT= Extra! 

SpectraComp and HyParComp come with 3 species compatibility.